Ensamble de macroinvertebrados bentónicos como indicadores de calidad de agua a través de la aplicación de un índice biótico modificado en un contexto espacio-estacional en una cuenca costera del sur de Chile

Tapia, J (Tapia, Jaime).Univ Talca, Inst Chem & Nat Resources, Talca, Chile.The water quality in a Southern Chile river was characterized using the Biotic Index in a spatio-seasonal context, to analyze the composition and distribution of benthic macroinvertebrates and their correlation with 14 physico-chemical parameters. Sampling was done over a year at 5 sampling stations in the basin of the Boroa River. A total of 77 taxa were recorded, with the most represented orders being the Ephemeroptera (43.30%), Diptera (24.16%) and Plecoptera (18.03%). The most abundant species were Meridialaris diguillina, Nousia maculata (Ephemeroptera) and Limnoperla jaffueli (Plecoptera). According to the modified FBI (Family Biotic Index), the findings indicated excellent, very good and good water quality, the latter quality being registered principally in winter. Likewise, the physico-chemical parameters indicated exceptional water quality. These results suggested a low level of anthropogenic impact in the basin. Applying the modified Hilsenhoff Biotic Index based on macroinvertebrates, the influence of seasonality on water quality was evident

Biometry and plasmatic stress-related parameters in brill (Scophthalmus rhombus)cultured at different stocking densities.

The effects of the stocking density on the physiological stress and biometric features of the brill were studied. Fish (491&plusmn;20 g) were cultured at three different stocking densities: 1; 5 and 15 Kg m-2 (LSD, MSD and HSD) during 5 weeks. Survival and several biometric, feeding and plasmatic parameters were assessed. Although final weight and specific growth rate decreased in higher densities, there were not significant differences between MSD and HSD. Differences for survival rate, feed efficiency, conversion index and feed intake were not detected among treatments. The minimum HSI was found in the HSD treatment, and condition factor varied inversely regards to stocking density. Plasma cortisol and osmolality were directly related to stocking density though the former was not significantly different among treatments. Plasma lactate and glucose significantly increased while stocking density rose. Nevertheless, free fatty acids did not vary among treatments, and triglycerides only decreased in LSD. This work was supported by Interreg Project 0251_ECOAQUA_5_E, financed by the EDRF (European Regional Development Fund).www.juntadeandalucia.es/agriculturaypesca/ifapa/ecoaqua. &nbsp;Se estudiaron los efectos de la densidad de cultivo sobre el estr&eacute;s fisiol&oacute;gico y par&aacute;metros biom&eacute;tricos en el parracho. Los peces (491&plusmn;20 g) fueron cultivados a tres densidades diferentes: 1; 5 y 15 Kg m-2 (LSD, MSD y HSD) durante 5 semanas. El peso final y crecimiento decrecieron con la densidad, pero no hubo diferencias significativas entre MSD y HSD. No se detectaron diferencias significativas entre tratamientos para supervivencia, eficiencia alimentaria, &iacute;ndice de conversi&oacute;n y tasa de ingesti&oacute;n. El HIS m&iacute;nimo fue para la HSD y el factor de condici&oacute;n vari&oacute; inversamente a la densidad. La osmolalidad y el cortisol, glucosa y lactato plasm&aacute;ticos estuvieron directamente relacionados con la densidad. Sin embargo, los &aacute;cidos grasos libres no variaron entre tratamientos. Este trabajo fue apoyado por el proyecto Interreg 0251_ECOAQUA_5_E , financiado por el FEDER (Fondo Europeo de Desarrollo Regional).www.juntadeandalucia.es/agriculturaypesca/ifapa/ecoaqua.</p

The biological basis of smoltification in Atlantic salmon

Chile is the second-largest producer of Atlantic salmon in the world, and the Chilean salmon production accounts for 27% of the world’s production. One important step of the productive cycle in freshwater is the smoltification process that prepares the fish for the marine life stage. This review describes the biological basis of smoltification in Atlantic salmon, with particular attention on branchial osmoregulatory adaptations. We also discuss some of the infectious diseases and problems in smoltification (two of the main causes of losses in Chilean aquaculture) that could be related from a physiological point of view

Comparative pan-genome analysis of Piscirickettsia salmonis reveals genomic divergences within genogroups

Indexación: Scopus.Piscirickettsia salmonis is the etiological agent of salmonid rickettsial septicemia, a disease that seriously affects the salmonid industry. Despite efforts to genomically characterize P. salmonis, functional information on the life cycle, pathogenesis mechanisms, diagnosis, treatment, and control of this fish pathogen remain lacking. To address this knowledge gap, the present study conducted an in silico pan-genome analysis of 19 P. salmonis strains from distinct geographic locations and genogroups. Results revealed an expected open pan-genome of 3,463 genes and a core-genome of 1,732 genes. Two marked genogroups were identified, as confirmed by phylogenetic and phylogenomic relationships to the LF-89 and EM-90 reference strains, as well as by assessments of genomic structures. Different structural configurations were found for the six identified copies of the ribosomal operon in the P. salmonis genome, indicating translocation throughout the genetic material. Chromosomal divergences in genomic localization and quantity of genetic cassettes were also found for the Dot/Icm type IVB secretion system. To determine divergences between core-genomes, additional pan-genome descriptions were compiled for the so-termed LF and EM genogroups. Open pan-genomes composed of 2,924 and 2,778 genes and core-genomes composed of 2,170 and 2,228 genes were respectively found for the LF and EM genogroups. The core-genomes were functionally annotated using the Gene Ontology, KEGG, and Virulence Factor databases, revealing the presence of several shared groups of genes related to basic function of intracellular survival and bacterial pathogenesis. Additionally, the specific pan-genomes for the LF and EM genogroups were defined, resulting in the identification of 148 and 273 exclusive proteins, respectively. Notably, specific virulence factors linked to adherence, colonization, invasion factors, and endotoxins were established. The obtained data suggest that these genes could be directly associated with inter-genogroup differences in pathogenesis and host-pathogen interactions, information that could be useful in designing novel strategies for diagnosing and controlling P. salmonis infection. © 2017 Nourdin-Galindo, Sánchez, Molina, Espinoza-Rojas, Oliver, Ruiz, Vargas-Chacoff, Cárcamo, Figueroa, Mancilla, Maracaja-Coutinho and Yañez.https://www.frontiersin.org/articles/10.3389/fcimb.2017.00459/ful

Preliminary study of the adaptation of Senegalese sole Solea senegalensis Kaup, 1858 to different salinities

Osmoregulatory and metabolic effects of adaptation to different water salinities (5, 15, 25, 42 and 55) were assessed during a period of 14 days in juveniles of Senegalese sole Solea senegalensis Kaup, 1858. The results showed a good capacity to adapt within the range of water salinities tested. Gill Na+,K+-ATPase activity showed a direct lineal relationship with regard to water salinity, whereas kidney Na+,K+-ATPase activity did not change. Plasma osmolality and ions levels did not present differences among the groups. Plasma cortisol, glucose and lactate were higher in the group maintained in the highest water salinity.El crecimiento del lenguado senegalés Solea senegalensis Kaup, 1858 está influido por diferentes variables, y una de ellas es la salinidad ambiental. En este trabajo, se estudian los efectos osmorreguladores y metabólicos de la aclimatación a distintas salinidades (5, 15, 25, 42 y 55) durante un periodo de 14 días en juveniles de esta especie. La actividad Na+,K+-ATPasa branquial presentó una relación lineal directa respecto a la salinidad ambiental, mientras que la actividad Na+,K+-ATPasa renal no experimentó variación alguna. Ni la osmolalidad ni los niveles de iones plasmáticos analizados mostraron diferencias significativas entre los diferentes grupos. El cortisol y los niveles de glucosa y lactato plasmático aumentaron en los ejemplares adaptados a la mayor salinidad. Los resultados mostraron una buena capacidad de aclimatación en el rango de salinidades ambientales analizado.Instituto Español de Oceanografí

Characterization of the peripheral thyroid system of gilthead seabream acclimated to different ambient salinities

Thyroid hormones are involved in many developmental and physiological processes, including osmoregulation. The regulation of the thyroid system by environmental salinity in the euryhaline gilthead seabream (Sparus aurata) is still poorly characterized. To this end seabreams were exposed to four different environmental salinities (5, 15, 40 and 55 ppt) for 14 days, and plasma free thyroid hormones (fT3, ff4), outer ring deiodination and Na+/K+ -ATPase activities in gills and kidney, as well as other osmoregulatory and metabolic parameters were measured. Low salinity conditions (5 ppt) elicited a significant increase in fT3 (29%) and ff4 (184%) plasma concentrations compared to control animals (acclimated to 40 ppt, natural salinity conditions in the Bay of Cadiz, Spain), while the amount of pituitary thyroid stimulating hormone subunit 13 (tshb) transcript abundance remained unchanged. In addition, plasma fT4 levels were positively correlated to renal and branchial deiodinase type 2 (dio2) mRNA expression. Gill and kidney T4-outer ring deiodination activities correlated positively with dio2 mRNA expression and the highest values were observed in fish acclimated to low salinities (5 and 15 ppt). The high salinity (55 ppt) exposure caused a significant increase in tshb expression (65%), but deiodinase gene expression (diol and dio2) and activity did not change and were similar to controls (40 ppt). In conclusion, acclimation to different salinities led to changes in the peripheral regulation of thyroid hormone metabolism in seabream. Therefore, thyroid hormones are involved in the regulation of ion transport and osmoregulatory physiology in this species. The conclusions derived from this study may also allow aquaculturists to modulate thyroid metabolism in seabream by adjusting culture salinity. (C) 2016 Elsevier Inc. All rights reserved.Socrates/Erasmus Grant from the European UnionUniversity of Cadiz [UCA 2009-074-FPI]Ministerio de Education y Ciencia, Spain [AGL2007-61211/ACU]FEDER, Spain [AGL2007-61211/ACU]Proyecto de Excelencia (Junta de Andalucia) [PO7-RNM-02843]Science Foundation (FCT) of Portugal [SFRH/BPD/89889/2012, SFRH/BPD/84033/2012]info:eu-repo/semantics/acceptedVersio

The involvement of thyroid hormones and cortisol in the osmotic acclimation of Solea senegalensis

Contains fulltext : 36596.pdf ( ) (Open Access)The peripheral conversion of the prohormone 3,5,3',5'-tetraiodothyronine (T4) to the biologically active 3,5,3'-triiodothyronine (T3), via enzymatic deiodination by deiodinases, is an important pathway in thyroid hormone metabolism. The aim of this study was to test if thyroid hormones and cortisol, as well as the outer ring deiodination (ORD) metabolic pathway, are involved in the osmoregulatory response of Senegalese sole (Solea senegalensis, Kaup 1858). We measured osmoregulatory and endocrine parameters in immature juveniles S. senegalensis acclimated to seawater (SW, 38 per thousand) and that were transferred and allowed to acclimate to different salinities (5 per thousand, 15 per thousand, 38 per thousand and 55 per thousand) for 17 days. An adjustment and a chronic regulatory period were identified following acclimation. The adjustment period immediately follows the transfer, and is characterized by altered plasma osmolalities. During this period, plasma cortisol levels increased while plasma free T4 (fT4) levels decreased. Both hormones levels returned to normal values on day 3 post-transfer. In the adjustment period, renal and hepatic ORD activities had increased concomitantly with the decrease in plasma fT4 levels in fishes transferred to extreme salinities (5 per thousand and 55 per thousand). In the chronic regulatory period, where plasma osmolality returned to normal values, plasma cortisol had increased, whereas plasma fT4 levels decreased in animals that were transferred to salinities other than SW. No major changes were observed in branchial ORD activity throughout the experiment. The inverse relationship between plasma cortisol and fT4 suggests an interaction between these hormones during both osmoregulatory periods while ORD pathway can be important in the short-term adjustment period.8 p